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STEMCELL Technologies Inc methylcellulose-based medium methocult h4034 optimum
Methylcellulose Based Medium Methocult H4034 Optimum, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/methylcellulose+medium/methocult/bio_rxiv__2025__07__15__664865-180-10-15
Average 90 stars, based on 1 article reviews
methylcellulose-based medium methocult h4034 optimum - by Bioz Stars, 2026-10
90/100 stars

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Suspension:

Article Title: Real‐Time, AI ‐Guided Photodynamic Laparoscopy Enhances Detection in a Rabbit Model of Peritoneal Cancer Metastasis
Article Snippet: For initial tumor induction, a donor rabbit bearing a VX2 xenograft on the hind leg was anesthetized with 0.25 mg/kg medetomidine (Tomidine; Provet, Turkey) intramuscularly and 2 mg/kg alfaxalone (Alfaxan multidose; Jurox, Rutherford, NSW, Australia) intravenously. .. One milliliter of a frozen VX2 tumor cell suspension (~1 × 10 6 cells) mixed in a 1:1 volumetric ratio with methylcellulose medium (STEMCELL Technologies, Seoul, Korea) was intramuscularly injected in the hind leg. ..

Injection:

Article Title: Real‐Time, AI ‐Guided Photodynamic Laparoscopy Enhances Detection in a Rabbit Model of Peritoneal Cancer Metastasis
Article Snippet: For initial tumor induction, a donor rabbit bearing a VX2 xenograft on the hind leg was anesthetized with 0.25 mg/kg medetomidine (Tomidine; Provet, Turkey) intramuscularly and 2 mg/kg alfaxalone (Alfaxan multidose; Jurox, Rutherford, NSW, Australia) intravenously. .. One milliliter of a frozen VX2 tumor cell suspension (~1 × 10 6 cells) mixed in a 1:1 volumetric ratio with methylcellulose medium (STEMCELL Technologies, Seoul, Korea) was intramuscularly injected in the hind leg. ..

Cell Culture:

Article Title: Targeting the IKZF1/BCL-2 axis as a novel therapeutic strategy for treating acute T-cell lymphoblastic leukemia.
Article Snippet: .. Cells were then cultured in methylcellulose medium (Stem Cell Technologies, Canada) at a density of 500 viable cells per well. .. After 10–14 days, colonies were stained with MTT solution (5 mg/mL) (Solarbio, China).

Colony Assay:

Article Title:
Article Snippet: In another case, 5 μM of P2X1 antagonist PPNDS (TOCRIS) was added to the methylcellulose medium and colony numbers were determined. .. For the colony formation assay with human primary AML cells, P2X1-knockdown (sh-P2X1 #1 and #2) and scrambled AML cells were seeded into a methylcellulose medium (Stemcell Technologies, #H4436) according to the manufacturer’s instruction, followed by the calculation of colony numbers and derived total cell numbers 7-10 days later. .. Human AML cell lines, such as THP-1 (ATCC), U937 (ATCC) or MV4-11 (ATCC), were cultured in the RPMI-1640 medium containing 10% FBS and subjected to the analysis of cell proliferation changes after P2X1 knockdown with its specific shRNAs (sh-P2X1 #1, #2 or scrambled) or after infected with empty vector (EV), WT or mutant P2X1 (S387A or T389A) plasmids at the indicated time points.

Derivative Assay:

Article Title:
Article Snippet: In another case, 5 μM of P2X1 antagonist PPNDS (TOCRIS) was added to the methylcellulose medium and colony numbers were determined. .. For the colony formation assay with human primary AML cells, P2X1-knockdown (sh-P2X1 #1 and #2) and scrambled AML cells were seeded into a methylcellulose medium (Stemcell Technologies, #H4436) according to the manufacturer’s instruction, followed by the calculation of colony numbers and derived total cell numbers 7-10 days later. .. Human AML cell lines, such as THP-1 (ATCC), U937 (ATCC) or MV4-11 (ATCC), were cultured in the RPMI-1640 medium containing 10% FBS and subjected to the analysis of cell proliferation changes after P2X1 knockdown with its specific shRNAs (sh-P2X1 #1, #2 or scrambled) or after infected with empty vector (EV), WT or mutant P2X1 (S387A or T389A) plasmids at the indicated time points.

Isolation:

Article Title: CD37 regulates the self-renewal of leukemic stem cells via integrin-mediated signaling in acute myeloid leukemia.
Article Snippet: .. 1,000 YFP+, c-kit+ cells isolated from leukemia mice transplanted with CD37fl/fl or CD37 / AML LSCs were plated on methylcellulose medium (STEMCELL, M3434) in a 6-well plate and incubated at 37 C for 10–14 days. ..

Incubation:

Article Title: CD37 regulates the self-renewal of leukemic stem cells via integrin-mediated signaling in acute myeloid leukemia.
Article Snippet: .. 1,000 YFP+, c-kit+ cells isolated from leukemia mice transplanted with CD37fl/fl or CD37 / AML LSCs were plated on methylcellulose medium (STEMCELL, M3434) in a 6-well plate and incubated at 37 C for 10–14 days. ..

In Vitro:

Article Title:
Article Snippet: .. In vitro colony formation unit assay and cell proliferation analysis Three thousand murine BM AML cells were seeded in a methylcellulose medium (Stem Cell Technologies, #M3534) according to the manufacturer’s information. ..

Article Title: The m 5 C methyltransferase NSUN2 promotes progression of acute myeloid leukemia by regulating serine metabolism.
Article Snippet: Subsequently, the plates were incubated at room temperature in the dark for 4 h. Samples were analyzed using a Victor2 multilabel counter (PerkinElmer) to detect the fluorescence at 485 nm (excitation)/535 nm (emission) for caspase3/7 activity. .. In vitro colony forming unit (CFU) and limiting dilution assays (LDAs) CFU and LDAs were carried out as previously described.15 For the CFU assay, human AML cells and MLL-AF9 leukemia cells were plated in methylcellulose medium (Stem Cell Technologies). ..

Colony-forming Unit Assay:

Article Title: The m 5 C methyltransferase NSUN2 promotes progression of acute myeloid leukemia by regulating serine metabolism.
Article Snippet: Subsequently, the plates were incubated at room temperature in the dark for 4 h. Samples were analyzed using a Victor2 multilabel counter (PerkinElmer) to detect the fluorescence at 485 nm (excitation)/535 nm (emission) for caspase3/7 activity. .. In vitro colony forming unit (CFU) and limiting dilution assays (LDAs) CFU and LDAs were carried out as previously described.15 For the CFU assay, human AML cells and MLL-AF9 leukemia cells were plated in methylcellulose medium (Stem Cell Technologies). ..



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STEMCELL Technologies Inc methylcellulose medium
Combination of Q/M-induced proapoptotic activity is p53-dependent, and the combination treatment impairs clonogenicity in FLT3 mutant/ TP53 WT leukemia blasts. A, MOLM-13-p53-Vec or MOLM-13-p53-KD cells were treated with increasing concentrations of single-agent milademetan or quizartinib or Q/M combination for 48 hours. Apoptosis was measured by FCM with annexin V staining. CIs were calculated using CalcuSyn software (version 2.0, PREMIER Biosoft). B, MOLM-13-p53-Vec or MOLM-13-p53-KD cells were treated with MOLM-13 and milademetan (60 nmol/L) or quizartinib (3 nmol/L) or the combination for 24 hours. Correlated signaling pathway proteins were measured with immunoblotting. C, Primary AML samples (three cases; harboring FLT3 mutant/ TP53 WT) and two normal BM samples from normal donors were treated with indicated concentrations of quizartinib and/or milademetan for 2 weeks in <t>methylcellulose</t> medium. CFU-GM colonies were counted, and the percentage of colony forming against the control group was calculated. The data were obtained from triplicated wells, and error bars are presented as mean ± SD. Asterisks indicate the level of statistical significance. *, P < 0.05; **, P < 0.01; ***, P < 0.001 as determined using a two-tailed unpaired t test. BAX, Bcl-2–associated X; CFU-GM, colony-forming unit granulocyte–macrophage; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NS, no statistical significance.
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Combination of Q/M-induced proapoptotic activity is p53-dependent, and the combination treatment impairs clonogenicity in FLT3 mutant/ TP53 WT leukemia blasts. A, MOLM-13-p53-Vec or MOLM-13-p53-KD cells were treated with increasing concentrations of single-agent milademetan or quizartinib or Q/M combination for 48 hours. Apoptosis was measured by FCM with annexin V staining. CIs were calculated using CalcuSyn software (version 2.0, PREMIER Biosoft). B, MOLM-13-p53-Vec or MOLM-13-p53-KD cells were treated with MOLM-13 and milademetan (60 nmol/L) or quizartinib (3 nmol/L) or the combination for 24 hours. Correlated signaling pathway proteins were measured with immunoblotting. C, Primary AML samples (three cases; harboring FLT3 mutant/ TP53 WT) and two normal BM samples from normal donors were treated with indicated concentrations of quizartinib and/or milademetan for 2 weeks in methylcellulose medium. CFU-GM colonies were counted, and the percentage of colony forming against the control group was calculated. The data were obtained from triplicated wells, and error bars are presented as mean ± SD. Asterisks indicate the level of statistical significance. *, P < 0.05; **, P < 0.01; ***, P < 0.001 as determined using a two-tailed unpaired t test. BAX, Bcl-2–associated X; CFU-GM, colony-forming unit granulocyte–macrophage; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NS, no statistical significance.

Journal: Clinical Cancer Research

Article Title: Synergistic Activity of Combined FLT3-ITD and MDM2 Inhibition with Quizartinib and Milademetan in FLT3 -ITD Mutant/ TP53 Wild-type Acute Myeloid Leukemias

doi: 10.1158/1078-0432.CCR-24-2764

Figure Lengend Snippet: Combination of Q/M-induced proapoptotic activity is p53-dependent, and the combination treatment impairs clonogenicity in FLT3 mutant/ TP53 WT leukemia blasts. A, MOLM-13-p53-Vec or MOLM-13-p53-KD cells were treated with increasing concentrations of single-agent milademetan or quizartinib or Q/M combination for 48 hours. Apoptosis was measured by FCM with annexin V staining. CIs were calculated using CalcuSyn software (version 2.0, PREMIER Biosoft). B, MOLM-13-p53-Vec or MOLM-13-p53-KD cells were treated with MOLM-13 and milademetan (60 nmol/L) or quizartinib (3 nmol/L) or the combination for 24 hours. Correlated signaling pathway proteins were measured with immunoblotting. C, Primary AML samples (three cases; harboring FLT3 mutant/ TP53 WT) and two normal BM samples from normal donors were treated with indicated concentrations of quizartinib and/or milademetan for 2 weeks in methylcellulose medium. CFU-GM colonies were counted, and the percentage of colony forming against the control group was calculated. The data were obtained from triplicated wells, and error bars are presented as mean ± SD. Asterisks indicate the level of statistical significance. *, P < 0.05; **, P < 0.01; ***, P < 0.001 as determined using a two-tailed unpaired t test. BAX, Bcl-2–associated X; CFU-GM, colony-forming unit granulocyte–macrophage; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NS, no statistical significance.

Article Snippet: The cells (0.2 × 10 6 ) were plated in methylcellulose medium (1 mL/well; cat.: 04435; STEMCELL Technologies Inc.) for 2 weeks, exposing them to the indicated drugs (in triplicate per condition).

Techniques: Activity Assay, Mutagenesis, Staining, Software, Western Blot, Control, Two Tailed Test